Dr. Daniel Zeve

Our group interrogates ISC function within the organoid culturing system using small molecule-driven differentiation, adenoviral and lentiviral over-expression and CRISPR knockout, qPCR, immunofluorescence staining, flow cytometry, hormone ELISAs, co-immunoprecipitation, RNA sequencing, single cell RNA sequencing, and SHARE-seq (combined single nuclear RNA sequencing and single nuclear ATAC sequencing).